Plasmid

Part:BBa_K4034011:Design

Designed by: Spyros Kanellopoulos   Group: iGEM21_Athens   (2021-10-17)


pADAPTED2t7


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 296
    Illegal EcoRI site found at 5533
    Illegal EcoRI site found at 6133
    Illegal PstI site found at 234
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 296
    Illegal EcoRI site found at 5533
    Illegal EcoRI site found at 6133
    Illegal PstI site found at 234
    Illegal NotI site found at 5544
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 296
    Illegal EcoRI site found at 5533
    Illegal EcoRI site found at 6133
    Illegal BglII site found at 2287
    Illegal BamHI site found at 290
    Illegal XhoI site found at 284
    Illegal XhoI site found at 5538
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 296
    Illegal EcoRI site found at 5533
    Illegal EcoRI site found at 6133
    Illegal PstI site found at 234
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 296
    Illegal EcoRI site found at 5533
    Illegal EcoRI site found at 6133
    Illegal PstI site found at 234
    Illegal NgoMIV site found at 704
    Illegal AgeI site found at 891
    Illegal AgeI site found at 1233
    Illegal AgeI site found at 1632
    Illegal AgeI site found at 3453
    Illegal AgeI site found at 3944
    Illegal AgeI site found at 3950
    Illegal AgeI site found at 4484
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

no


Source

no

References